Clonal Lines and Chromosome Manipulation for Aquaculture Research and Production

Abstract

Chromosome set manipulation in many aquaculture species has been used as a tool for monosex production, production of polyploids to induce sterility or to improve growth, and production of clonal lines for applied and basic research in quantitative trait analysis, genome architecture, and evolution. Several previous studies have extensively reviewed the utility and status of chromosome set manipulation in aquaculture (Tave 1993; Pandian and Koteeswaran 1998; Arai 2001; Felip et al. 2001; Hulata 2001), and more recently, the use of these techniques for the development of clonal lines in fishes (Komen and Thorgaard 2007). In this chapter, an overview is provided for the basic methodologies used for chromosome set manipulation in fish and shellfish aquaculture species, with special attention paid to recent advances in the use of these techniques for commercial aquaculture production, or improvement of strains used in aquaculture. Finally, the utilization of clonal lines for linkage mapping, quantitative trait analysis, and aquaculture production is reviewed, with a forward perspective on the use of clonal lines and chromosome set manipulation for future applications in aquaculture.