UMT-IR Home
View Item
81,733 Item Views
  • 5,303 Collection Views Page Views
  • 4,801 Community Views
  • 2,031 User Logins
  • 370,530 Searches Performed
  • 3,993 All Items

Preparation of Eel (Monopterus Albus) Protein Hydrolysate Using Alcalase® And Its Physicochemical, Antioxidant And Anticancer Properties

Show full item record

Title: Preparation of Eel (Monopterus Albus) Protein Hydrolysate Using Alcalase® And Its Physicochemical, Antioxidant And Anticancer Properties
Author: Nura Ruhaya Binti Abdul Halim
Abstract: The study aims to determine the optimum condition of eel protein hydrolysate (EPH) produced using Alcalase® and its physicochemical, antioxidant and anticancer properties. The hydrolysis condition was optimized using Response Surface Methodology (RSM) by applying four factors (time, temperature, pH, Alcalase® concentration), 3-levels Central Composite Design (CCD) with six central points. The chemical and amino acid compositions, structural properties, solubility, emulsifying and foaming properties, water holding and fat binding capacity of EPH were determined. The EPH was separated through ultrafiltration membranes (10 kDa, 5 kDa and 3 kDa). The ferric thiocyanate, thiobarbituric reactive substance, reducing power, ferrous ion chelating activity and 1,2-diphenylpicrylhydrazyl radical scavenging activity of fractionated EPH were also determined. The anticancer activity was determined using MCF-7 cell lines. In order to obtain the optimum yield, degree of hydrolysis (DH) and DPPH activity, the optimum hydrolysis condition suggested was 84.02 min, 50.18°C, pH 7.89 and 2.26% Alcalase® concentration
URI: http://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/14255
Date: 2017-10


Files in this item

Files Size Format View
Full Text SH 351 .E4 N8 2017.pdfBlocked 1.074Mb PDF View/Open
Abstract SH 351 .E4 N8 2017.pdf 97.30Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record

Search UMT-IR

Browse

My Account